1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pAIDA1

   Plasmid

   #79180

   Purpose

   A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

   Depositor

   Gen Larsson

   Article

   Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

   Insert

   Adhesin Involved in Diffuse Adherence
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR26 iBFP Dest

   Plasmid

   #74284

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-FLAG

   Plasmid

   #183511

   Purpose

   Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU294

   Plasmid

   #167689

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC7

    Plasmid

    #118876

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU295

    Plasmid

    #167694

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLP-hyg

    Plasmid

    #87831

    Purpose

    Expresses FLP recombinase with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFlp-Tc

    Plasmid

    #222336

    Purpose

    flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB )
    flp (flp )
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrEno

    Plasmid

    #115474

    Purpose

    Eno-promoter driven expression vector for protein production in Trichoderma reesei

    Depositor

    Steve Decker

    Article

    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

    Insert

    Cel7A
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Introns have been removed

    Promoter

    Trichoderma reesei Enolase (Eno) promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH75

    Plasmid

    #128224

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p201H Cas9

    Plasmid

    #59176

    Purpose

    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    hph
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge