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Plasmid#134702PurposeLentiviral expression of human MEFV after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationWTPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV Rac
Plasmid#50535DepositorInsertsmall GTPase (RAC1 Human)
UseTags2X VSVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A2
Plasmid#132126PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A2 (SLC2A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
UseTagseGFPExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hFGD5
Plasmid#50760Purposeexpression of EGFP-tagged human FGD5DepositorInsertFGD5 (FGD5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAFi-dHdrA
Plasmid#184907Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferriduransDepositorInsertCm-sgRNA-dCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
P70a-deGFP-sc101-kan
Plasmid#184841PurposeExpression of deGFPDepositorInsertgreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpromoter OR2-OR1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
OKSIM
Plasmid#24603DepositorUseCre/Lox and LentiviralTagsExpressionMammalianMutationOct4-2A-KLF4-2A-Sox2-IRES-MycPromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
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TFORF0727
Plasmid#141674PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFP36L2 (ZFP36L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
UseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TL024_lenti_hSyn:HaloTag-GluA1
Plasmid#192517PurposeExpression of AMPA GluA1 with N terminal HaloTag fusionDepositorInsertHaloTag-GluA1
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterhSynAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC65
Plasmid#118865Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-SNAPf-CCR5TC
Plasmid#135536PurposeEncodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseTagsSNAPfExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dsup pLVX-Puro
Plasmid#180635PurposeExpresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio toleranceDepositorInsertDsup
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-C
Plasmid#30224DepositorInsertrhVitronectin with C terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478PromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_ERN1_WT
Plasmid#82115PurposeGateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.DepositorInsertERN1 (ERN1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VHC
Plasmid#112618Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2DepositorInsertsUseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-Ostir-neo
Plasmid#161973PurposeExpresses Ostir and G418 resistance gene in mammalian cellsDepositorInsertOstir-3xHA-p2a-neoR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG12DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK3R5
Plasmid#20576DepositorInsertPIK3R5 (PIK3R5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
UseTagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHJ17
Plasmid#48356PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_nucl
Plasmid#113931PurposePlasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc_nucl
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::ZeoR-bGHpA
Plasmid#62442PurposeMXS_chaining vector with PGK::ZeoR-bGHpADepositorInsertresistance cassette against Zeocin with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159553PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only