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Showing: 1381 - 1410 of 156661 results

  2. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  3. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  7. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Article
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158230-plasmid-map-sequence-id-317503

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  16. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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  19. pCINEO-3xFlag-hRIPK3

    Plasmid
    #112495
    Purpose
    Mammalian expression of 3xFlag-hRIPK3
    Depositor
    Junying Yuan
    Insert
    RIPK3 (RIPK3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112495-plasmid-map-sequence-id-214535

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  23. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  25. MC4R-Tango

    Plasmid
    #66430
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC4R (MC4R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66430-plasmid-map-sequence-id-314166

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  29. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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Showing: 1381 - 1410 of 156661 results