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Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mTagBFP2 rat PGK1 shRNA
Plasmid#222869PurposeExpresses mTagBFP2 along with an shRNA against rat PGK1DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-Kan
Plasmid#44900PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG1160 mCherry-FIREtag
Plasmid#205164PurposeExpresses mCherry -FIREtag in mammalian cellsDepositorInsertmCherry-FIREtag
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTcI-Wasabi
Plasmid#86101PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcIDepositorInsertsTcI
mWasabi
UseTags6xHisExpressionBacterialMutationA67TPromoterpR/pL and pR/pL, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationV93QPromoterT7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 JN.1
Plasmid#216526PurposeEncodes SARS-CoV-2 spike for JN.1 pseudovirus productionDepositorInsertSARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO EYFP
Plasmid#27056DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10-P2A-GFP
Plasmid#162509PurposeExpress ABE7.10-P2A-GFP by CMV promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-Bcl-XL
Plasmid#46972Purposelentiviral expression of Bcl-XLDepositorInsertBCL2L1 (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC0078 RESCUE
Plasmid#130661PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/DepositorInsertdRanCas13b-ADAR2dd (RESCUE)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_FLUAV_HA
Plasmid#207273PurposeMammalian expression of Influenza A virus HA proteinDepositorInsertFLUAV_HA (HA Influenza A virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
UseTagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-gRNA-EF1a-EGFP-CAAX
Plasmid#167936PurposegRNA entry vectorDepositorInsertU6-gRNA backbone
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-N1
Plasmid#53976PurposeLocalization: N1 Cloning Vector, Excitation: 487, Emission: 509DepositorTypeEmpty backboneUseTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsp27 wt FL
Plasmid#17444DepositorInsertsmall heat shock protein27 (HSPB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only