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Plasmid#71640PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methioninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK688
Plasmid#71641PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to argininePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK695
Plasmid#71644PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges histidine-17 to tryptophanPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK696
Plasmid#71645PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges methionine-15 to tryptophanPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK607
Plasmid#71626PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to cysteinePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK617
Plasmid#71627PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to alaninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK643
Plasmid#71628PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to threoninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK644
Plasmid#71629PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to aspartatePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK125
Plasmid#73336PurposeEncodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 1-327PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK553
Plasmid#72452PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK530
Plasmid#72511PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)DepositorInserthypothetical protein in purSQL operon
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK533
Plasmid#72514PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK575
Plasmid#72526PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK620
Plasmid#72537PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK622
Plasmid#72538PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK590
Plasmid#71698PurposeProduces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)DepositorInsertthioredoxin reductase 1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK648
Plasmid#71611PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges phenylalanine-79 to tryptophanPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRP-iSL(MCS1+2)
Plasmid#69245PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD450
Plasmid#66153Purposecloning of E coli E2348/69 PerADepositorInsertperA
UseCloning vectorTagsExpressionMutationPromoternoneAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only