pJK125
(Plasmid
#73336)
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PurposeEncodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 73336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6313
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameelectron transfer flavoprotein
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Alt namefdxB
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Alt nameLH57_19385
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SpeciesMycobacterium tuberculosis H37Rv
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Mutationencodes residues 1-327
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GenBank IDAIR16344.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains putative integral-membrane fatty acid desaturase domain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK125 was a gift from T. J. Kappock (Addgene plasmid # 73336 ; http://n2t.net/addgene:73336 ; RRID:Addgene_73336)