-
Plasmid#201955PurposeMammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A on FnCas9, V82S, V106W, Q154R on mutant TadAPromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-G671R-N865K
Plasmid#203699Purposeexpresses ATP10B with the pathogenic mutations G671R and N865KDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationG671R, N865KPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP
Plasmid#194016PurposeExpresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFPDepositorInsertssgRNA(Oxtr.2)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromotercmb and u6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-79
Plasmid#197397PurposeExpresses SUMO-tagged mouse METAP1, residues 1-79DepositorInsertMETAP1 1-79 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-59
Plasmid#197396PurposeExpresses SUMO-tagged mouse METAP1, residues 1-59DepositorInsertMETAP1 1-59 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192235Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192243Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192244Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1
Plasmid#187288PurposeExpress pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63LDepositorUseTagsmCherryExpressionMammalianMutationAH A740D, E758K, RhoA Q63LPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+10subs
Plasmid#167176PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+10R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged A at 112 to T; E at 116 to D; I at 135 to…PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+12subs
Plasmid#167177PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+12R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R; A at 112 to T; E at 116to …PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v4
Plasmid#173788PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 4DepositorInsertSpike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
miR3 HsATP10B
Plasmid#171824Purposetransfer plasmid for lentiviral vector production with miR for Hs ATP10BDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR7 MmATP10B
Plasmid#171825Purposetransfer plasmid for lentiviral vector production with miR for Mm ATP10BDepositorInsertATP10B (Atp10b Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR5 MmATP10B
Plasmid#171791Purposetransfer plasmid for lentiviral vector production with miR for Mm ATP10BDepositorInsertATP10B (Atp10b Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR2 HsATP10B
Plasmid#171790Purposetransfer plasmid for lentiviral vector production with miR for Hs ATP10BDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut-miR-375-mutA-E
Plasmid#53714PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutationsDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationMutation on the stop codon of firefly luciferase …PromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag VRK3
Plasmid#20664DepositorInsertVRK3 (VRK3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-GFP
Plasmid#46148DepositorTypeEmpty backboneUseTagsGFPExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only