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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2

Plasmid

#135504

Purpose

Mammalian expression of myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-CD19 puro

Plasmid

#196634

Purpose

Express CD19

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

CD19 (CD19 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MscL(G22S)-c-term-cpGFP-ERexp

Plasmid

#195346

Purpose

Control plasmid for the MscL tension biosensor

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

MscL(G22S)-c-term-cpGFP-ERexp

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVd2

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE31

Plasmid

#90373

Purpose

Hippo - TEAD1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Hippo

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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