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pAC1808_pmax-RBFOX1N-FRB-C

Plasmid

#118641

Purpose

Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-FRB-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> :dTomato:IRES:Puro

Plasmid

#110832

Purpose

Expresses dTomato (dT) red fluorescent reporter.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

dTomato Tag

Use

Non-viral gene expression vector

Tags

IRES

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAC1807_pmax-dCasRx-FKBP

Plasmid

#118640

Purpose

Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-FKBP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al Elife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNOC-RLUC

Plasmid

#98142

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

*please see depositor comment below

Promoter

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP4

Plasmid

#80660

Purpose

Encodes the synthetic coiled coil peptide SYNZIP4

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-3XAP-NLG1

Plasmid

#43923

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

3XAP-NLG1

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP1

Plasmid

#80647

Purpose

Encodes the synthetic coiled coil peptide SYNZIP1

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 5-6

Plasmid

#54364

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP3

Plasmid

#80659

Purpose

Encodes the synthetic coiled coil peptide SYNZIP3

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pGWT35S-RBCS1a(1–79)-TagRFP

Plasmid

#182586

Purpose

Transient expression of RBCS1a(1–79)-TagRFP in plant cell (Plastid/Chloroplast)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

RBCS1a(1–79)

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rac1 DN

Plasmid

#52234

Purpose

Drosophila expression of YFP-Rac1 dominant negative mutant

Depositor

Matthew Scott

Insert

Rac1 (Rac1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T17N; DN mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Tags

Expression

Mutation

Promoter

35S

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Use

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

adh1

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1658

Plasmid

#119179

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGAP

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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