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Showing: 9301 - 9320 of 34751 results

  1. pET28a-FKBP-TRIF(Nterm)

    Plasmid
    #221334
    Purpose
    Overexpression of TRIF Nterm domain in bacteria
    Depositor
    Jonathan Kagan
    Insert
    TRIF Nterm domain (Ticam1 Mouse)
    Use
    Tags
    6xHis-4xFKBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221334-plasmid-map-sequence-id-438969

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  2. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  3. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

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  4. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

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  5. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  6. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

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  7. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  8. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid
    #218194
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3
    Depositor
    Florence Pojer
    Insert
    EndoF3
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-329
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218194-plasmid-map-sequence-id-431796

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  9. pET-29b(+)_His-CBD-EmEndoF1

    Plasmid
    #218195
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1
    Depositor
    Florence Pojer
    Insert
    EndoF1
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 51-339
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218195-plasmid-map-sequence-id-431792

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  11. Peroxisomes-FAST-IRES-iRFP670

    Plasmid
    #216347
    Purpose
    For testing localization of pFAST and iRFP670 proteins to peroxisomes
    Depositor
    Alison Tebo
    Insert
    pFAST_SKL-IRES-iRFP670_PXMP2
    Use
    Tags
    myc for FAST protein only
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216347-plasmid-map-sequence-id-427406

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  12. Mito-FAST-IRES-iRFP670

    Plasmid
    #216346
    Purpose
    For testing localization of pFAST and iRFP670 proteins to mitochondria
    Depositor
    Alison Tebo
    Insert
    Cox8_pFAST-IRES-Tomm20_iRFP670
    Use
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216346-plasmid-map-sequence-id-427397

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  13. ER-FAST-IRES-iRFP670

    Plasmid
    #216344
    Purpose
    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum
    Depositor
    Alison Tebo
    Insert
    PRL_pFAST-IRES-CANX_iRFP670
    Use
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216344-plasmid-map-sequence-id-427396

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  14. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

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  15. PgyrA_gfp

    Plasmid
    #212146
    Purpose
    Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212146-plasmid-map-sequence-id-420759

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  16. pmVen-C16orf59

    Plasmid
    #211423
    Purpose
    mammalian expression of human C16orf59 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211423-plasmid-map-sequence-id-420526

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  17. pmCer-C16orf59

    Plasmid
    #211422
    Purpose
    mammalian expression of human C16orf59 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211422-plasmid-map-sequence-id-420524

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  18. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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  19. pENTR3cDual-Wnk

    Plasmid
    #203976
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203976-plasmid-map-sequence-id-403303

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  20. pDONR221-deltaUBA-short-UBASH3A-Ctag

    Plasmid
    #195397
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195397-plasmid-map-sequence-id-388659

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Showing: 9301 - 9320 of 34751 results