Showing: 9301 - 9320 of 12981 results
-
Plasmid#169920PurposeExpression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-N57
UseCRISPRTagsExpressionMutationN57 is the PAI domain of SB100XPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v1)-Blast
Plasmid#170067PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistanceDepositorInsertKRAB-dCas9
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-Puro
Plasmid#170069PurposeExpresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoterDepositorInsertCas9 guide RNA scaffold with the F+E scaffold modification
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA1
Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterDpy-30AvailabilityAcademic Institutions and Nonprofits only -
pSpG-PPE
Plasmid#170130PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9-SpG(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…Promotermaize Ubiquitin-1AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PPE-V2
Plasmid#170131PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3AvailabilityAcademic Institutions and Nonprofits only -
pOsU3
Plasmid#170132PurposeFor sgRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUbi-MAD7
Plasmid#170133PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1AvailabilityAcademic Institutions and Nonprofits only -
pMAD7-crRNA-2
Plasmid#170135PurposeFor crRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry
Plasmid#170184PurposeExpression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.DepositorInsertsSa163 RT
Retron Sa163 msr-msd
BFP-to-GFP conversion donor template An
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCBh and hU6AvailabilityAcademic Institutions and Nonprofits only -
pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry
Plasmid#170185PurposeExpression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.DepositorInsertsEc86 RT
Retron Ec86 msr-msd
BFP-to-GFP conversion donor template An
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCBh and hU6AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Tyr
Plasmid#170293PurposeA knock-out vector for the mouse tyrosinase.DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 9301 - 9320 of 12981 results