-
Plasmid#212667PurposeExpress tagged DHBDepositorInsertDHB (HELB Human)
UseLentiviralTagsmTurq2ExpressionMutationPromoterEF-1aAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB-SaCas9
Plasmid#105998PurposeSaCas9 expression vectorDepositorInsertCas9 nuclease
UseCRISPR and LentiviralTagsnoneExpressionMammalianMutationnonePromotershort EF1alphaAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk3a
Plasmid#208985PurposeRiboprobe generationDepositorInsertkcnk3a (kcnk3a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSOX9-5
Plasmid#40646DepositorInsertsh-mSOX9-5 (Sox9 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pRRG36-ChAT
Plasmid#99069PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)DepositorInsertChAT (choline acetyltransferase) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-E2F-1 wt-pRcCMV
Plasmid#21667DepositorInsertE2F-1 (E2F1 Human)
UseTagsHA-tagExpressionMammalianMutationwild type-PromoterAvailable sinceSept. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
GST-TACC3-His6
Plasmid#69106PurposeFor bacterial expression of recombinant human TACC3 tagged with GST and His.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRTagsExpressionMutationPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorInsertTubulin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRb01_syn20
Plasmid#49704DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf01_syn20Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-bjGFP
Plasmid#67032PurposePlasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Mito-7
Plasmid#57401PurposeLocalization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertMito (COX8A Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLQ-Pxyl/tet-cas9-Pj23119-sgRNA
Plasmid#92121PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-pj23119
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG HDAC5 WT
Plasmid#32213DepositorInsertHDAC5 (HDAC5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only