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Plasmid#149573Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pBbdCas9S(RBSmut)_arr2
Plasmid#149575Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B(RBSmut)_arr2
Plasmid#149579Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(RBSmut)_arr2
Plasmid#149580Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM41991
Plasmid#153219PurposeLevel M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in AgrobacteriumDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA065
Plasmid#128517PurposeExpresses Ber e 1 in PichiaDepositorInsertBer e 1 a allergenic 2S albumin from Brazil nut
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GDF11-V5 blast
Plasmid#83098PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorInsertGDF11 (GDF11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-BsCas12b
Plasmid#121952PurposeE. coli expression, protein purificationDepositorInsertBsCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-U61-SapI
Plasmid#112808PurposegRNA cloning vector for expressing 1-3 gRNAs in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only