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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSC2

Plasmid

#91180

Purpose

T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting NCR genes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVL44 - pVND7>>

Plasmid

#116010

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GAL-p300-230-670

Plasmid

#89099

Purpose

mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 230-670

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-2-300

Plasmid

#89098

Purpose

mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 2-300

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
LLP251 pEF1a-UNC5C-4-SunTag

Plasmid

#100940

Purpose

TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for UNC5C, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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STT3A pmRFP (663)

Plasmid

#61997

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mPapaya1-Actin-C-18

Plasmid

#56400

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Nrp2.5-Fc-His

Plasmid

#72102

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1601-1900

Plasmid

#89102

Purpose

mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1601-1900

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV2-SMAC-HA-eGFP

Plasmid

#67488

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Use

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only