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Plasmid#162008PurposePIK3C2gamma shRNADepositorInsertPIK3C2G (PIK3C2G Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA SNX9 KD1
Plasmid#162014PurposeSNX9 shRNADepositorInsertSNX9 (SNX9 Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA SNX9 KD2
Plasmid#162015PurposeSNX9 shRNADepositorInsertSNX9 (SNX9 Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-tag RFPt- Rab11a WT
Plasmid#161992PurposeRetroviral expression vector containing Rab11a tagged with RFPDepositorInsertRAB11A (RAB11A Canis Lupus)
UseRetroviralTagsRFPExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PIK3C2A KD5
Plasmid#162006PurposePIK3C2alpha shRNADepositorInsertPIK3C2A (PIK3C2A Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PTEN KD4
Plasmid#162010PurposePTEN shRNADepositorInsertPTEN (PTEN Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PflaBS-sgRNAflaB
Plasmid#149643Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PflaBSAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_arr2
Plasmid#149582Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826L-sgRNAflaB
Plasmid#149648Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0826LAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F/V39L)
Plasmid#136610PurposeExpresses GB1 WT double mutant (A34F/V39L)DepositorInsertGB1 WT double mutant (A34F/V39L)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34F - V39LPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only