Addgene

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pMUT2-pCadBA-mCherry

Plasmid

#192865

Purpose

pMUT2 based vector for sensing pH in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

pH sensitive promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCag FlpO-2A-Cre EV

Plasmid

#129419

Purpose

episomal expression of FlpO and Cre recombinases

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

FlpO-2A-Cre

Use

Cre/Lox and Unspecified; Episomal expression vect…

Tags

Expression

Mammalian

Mutation

Promoter

CMV/Chick β-actin (CAG)

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-FAST-p10

Plasmid

#89152

Purpose

Express p10-FAST in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

NBV p10-FAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry empty

Plasmid

#80975

Purpose

Cas9, mCherry, and sgRNA expression

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-EGFP

Plasmid

#164092

Purpose

expression of EGFP-tagged proteins under CAG promoter

Depositor

Maik Hintze

Insert

EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME2f-mRuby2-ST

Plasmid

#170160

Purpose

Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-ABE8e(V106W)

Plasmid

#198533

Purpose

Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd-V106W

Plasmid

#193839

Purpose

Express TadCBEd-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd V106W-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-VPS15-TSF

Plasmid

#203563

Purpose

Mammalian expression of VPS15 with TwinStrep-Flag tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

VPS15 (PIK3R4 Human)

Use

Tags

TwinStrep Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-ATG14

Plasmid

#203567

Purpose

Mammalian expression of mCherry tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG14

Plasmid

#203566

Purpose

Mammalian expression of GST tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SspB-mScarlet-I

Plasmid

#178521

Purpose

Expresses SspB-mScarlet-I in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

SspB-mScarlet-I

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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