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Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNeo3/Cre
Plasmid#89653PurposeExpresses a Neomycin-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Neomycin
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_sgRNA
Plasmid#68422PurposeTransient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertGluc sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ158
Plasmid#69307PurposeGateway entry vector with hSpCas9 (Human codon optimized)DepositorInserthSpCas9 (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationNLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A8
Plasmid#99882PurposeRecipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.DepositorTypeEmpty backboneUseTALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-IVS-Syn21-GFP-P10
Plasmid#104614PurposeExpresses GFP upon binding of TALE3 to VAS3DepositorInsert3xVAS3
UseTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-IVS-Syn21-GFP-P10
Plasmid#104613PurposeExpresses GFP upon binding of TALE1 to VAS1DepositorInsert3xVAS1
UseTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-pA
Plasmid#220752PurposeExpression of Sun1GFP for nuclei isolationDepositorInsertSun1-GFP-WPRE
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
nCas9-NG_P2A-mCherry
Plasmid#219934PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC293-P4B1
Plasmid#203134PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293DepositorInsertEGFP/BC293
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC170-P2G2
Plasmid#203011PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC170DepositorInsertEGFP/BC170
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC157-P2F1
Plasmid#202998PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC157DepositorInsertEGFP/BC157
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC120-P2B12
Plasmid#202961PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC120DepositorInsertEGFP/BC120
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC121-P2C1
Plasmid#202962PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC121DepositorInsertEGFP/BC121
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC282-P3H6
Plasmid#203123PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC282DepositorInsertEGFP/BC282
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC232-P3D4
Plasmid#203073PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC232DepositorInsertEGFP/BC232
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only