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Plasmid#172585PurposeFor transient expression of N-terminal GFP-tagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCML 961
Plasmid#98848PurposepTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).DepositorInserts5' UTR + first 100 nucleotides of coding sequence of the aidB gene
csrA
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-GFP
Plasmid#39291DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4b (mEosFP4b-V69T)
Plasmid#99226PurposeBacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).DepositorInsertpr-mEos4b
UseTagsExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A
Plasmid#83461PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, R4113APromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2s
Plasmid#118036PurposeGABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminusDepositorInsertGABRG2 (Gabrg2 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P531A-pBabe-puro
Plasmid#26091DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA with point mutation P531APromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LplA(AAG)
Plasmid#61820PurposeContains GFP tag for direct live-cell fluorescence imaging of the resofurin ligaseDepositorInsertE. coli lipoic acid ligase
UseTagsGFPExpressionMammalianMutationE20A, F147A, H149GPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myc-delta
Plasmid#118037PurposeGABAA receptor expression (delta subunit) Myc-tag near N-terminusDepositorInsertGABRD (Gabrd Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode
Plasmid#170229PurposeBarcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertRHEB (RHEB Human)
UseLentiviralTagsFLAGExpressionMutationQ64LPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GFP
Plasmid#41616DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1
Plasmid#172581PurposeFor transient expression of C-terminal BirA-tagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE
Plasmid#118279PurposeExpresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cellsDepositorInsertsmRuby2
ChR2(H134R)
UseAAV and Cre/LoxTagsSV40 NLS and YFPExpressionMutationPromoterEF1 alphaAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GST
Plasmid#39287DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT250_(pCA-G-intron(Neo)-tTA2)
Plasmid#36876DepositorInsertsGFP
tTA2
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)
Plasmid#83465PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(LNL)
Plasmid#36907DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4DdeltaC
Plasmid#51602Purposeexpresses Sema4D lacking C terminusDepositorInsertSema4DdeltaC (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationLast 69 amino acids of Sema4D C-terminus are dele…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only