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Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
(K) tNOS (S)_T3 (SBE120)
Plasmid#195010Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)DepositorInsert(K) tNOS (S)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(H) tNOS (I) _T2 (SBE119)
Plasmid#195006Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)DepositorInsert(H) tNOS (I)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) HYG marker(R) (SBE264)
Plasmid#195013Purposehygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+HYG+tGPD
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(E) tNOS (F) _T1 (SBE118)
Plasmid#194997Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)DepositorInsert(E) tNOS (F)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) BLE marker(R) (SBE262)
Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
H446 EF1a RA-ABE6.3 p2A Puro
Plasmid#139104PurposeLentiviral expression of ABE6.3.DepositorInsertABE RA6.3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-eGFP
Plasmid#126720Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTags6xHis,MycExpressionYeastMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1585588
Plasmid#62620PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACB108
Plasmid#222207PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tagDepositorInsertfghA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB109
Plasmid#222208PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tagDepositorInsertfghA (K10N)
UseTags6x HisExpressionBacterialMutationChanged Lys10 to AsnPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB110
Plasmid#222209PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tagDepositorInsertfghA (G258D)
UseTags6x HisExpressionBacterialMutationChanged Gly258 to AspartatePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_A28C_R
Plasmid#200184PurposeWT PA14 I-F Leader and A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (A28C)
UseTagsExpressionBacterialMutationA28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F)pGPD(G)_P2 (SBE110)
Plasmid#194992Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)DepositorInsert(F) pGPD (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pADH2 (J) P3 (SBE114)
Plasmid#187629Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)DepositorInsert(I) pADH2 (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pADH2 (G)_P2 (SBE113)
Plasmid#187628Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)DepositorInsert(F) pADH2 (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only