Addgene

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OphA-delta C6

Plasmid

#127679

Purpose

Bacterial expression of His-tagged OphA-delta C6

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GB4668

Plasmid

#215272

Purpose

Module for the expression of the N. nambi HISPS gene under the ABA- responsive MAPKKK18 promoter and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

pMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K63R)-MCS

Plasmid

#212727

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K48R)-MCS

Plasmid

#212726

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo-GFP

Plasmid

#212720

Purpose

Bacterial expression of His6-CUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72K

Plasmid

#127683

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72K

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72K

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72K

Promoter

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C18

Plasmid

#127678

Purpose

Bacterial expression of His-tagged OphA-delta C18

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C18

Use

Tags

His8

Expression

Bacterial

Mutation

delta C18

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y63F

Plasmid

#127680

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y63F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y63F

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y63F

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y66F

Plasmid

#127681

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y66F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y66F

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y66F

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72A

Plasmid

#127682

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y76F

Plasmid

#127684

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y76F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y76F

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y76F

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OpHA-delta C6-Y98A

Plasmid

#127685

Purpose

Bacterial expression of His-tagged OpHA-delta C6-Y98A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OpHA-delta C6-Y98A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y98F

Plasmid

#127686

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y98F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y98F

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98F

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Q172A

Plasmid

#127687

Purpose

Bacterial expression of His-tagged OphA-delta C6-Q172A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Q172A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Q172A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 901 - 920 of 3431 results

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