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Plasmid#175787PurposeExpresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tagDepositorInsertSidK (1-278)
UseTags3xFLAG and 6xHis TEVExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
UseTags6x HIS and TEVExpressionBacterialMutationC78I, I92V, K116N, Q134E, R283W, C287N, V293S, G3…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-ires-Luc
Plasmid#63576PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.DepositorInsertStop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_AOX-FLAG
Plasmid#177984PurposeAll-in-one dox-inducible expression of Alternative OxidaseDepositorInsertAOX
UseLentiviralTagsFLAGExpressionMammalianMutationCodon optimized cDNA for expression in human cellsPromoterTREAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER WT
Plasmid#49498PurposeExpresses HA-tagged ER wild-type in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-col4a1
Plasmid#160317PurposecfSGFP2-tagged collagen4a1 for mammalian expressionDepositorInsertCOL4A1 (COL4A1 Human)
UseTagscfSGFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorInsertADAR2 (ADARB1 Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 WT
Plasmid#12605DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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V5-TurboID-NES_pCDNA3
Plasmid#107169Purposeexpresses V5-tagged TurboID in the mammalian cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-OVA
Plasmid#64599PurposeExpression of chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_Gal4VP64
Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorUseTagsExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha 2xmcp HALO
Plasmid#64540PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.DepositorInsertMCP
UseLentiviralTagsHA, HALO, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3m
Plasmid#208702PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP
Plasmid#11150PurposeMammalian expression vector for expression of GFP (CAG promoter)DepositorHas ServiceCloning Grade DNAInsertEGFP from Aequorea victoria
UseTagsExpressionMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TFAM-GFP
Plasmid#113704PurposeFluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selectionDepositorInsertTFAM (Tfam Mouse)
UseRetroviralTagseGFPExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-8his
Plasmid#145145PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRia-v2
Plasmid#84832PurposeCRISPRi/a V2 library parental plasmidDepositorInsertssgRNA
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_C1
Plasmid#85042PurposeMammalian expression of mScarletDepositorHas ServiceCloning Grade DNAInsertmScarlet
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-MCS--4x6T-WPRE
Plasmid#196417PurposeEmpty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO1A
Plasmid#153141PurposeExpression of Flag tagged human FOXO1A in mammalian cellsDepositorInserthuman FOXO1 (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-Mut-T-Luc
Plasmid#60490PurposeContains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988DepositorInsertHMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
UseLuciferaseTagsLuciferase reporterExpressionMammalianMutationFour point mutations (G->C or A->C) in the …PromoterPartial HMG-CoA synthase promoter (-324/-225) fus…Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP
Plasmid#11085DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-hygro_GFP
Plasmid#162075Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistanceDepositorInsertsCas9-2a-hygromycin B phosphotransferase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 myc PGC-1 alpha
Plasmid#10974PurposeMammalian expression of PGC-1a with myc and his tagsDepositorInsertPGC-1 alpha (PPARGC1A Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-EGFP
Plasmid#27082PurposeIntegration-free (episomal) expression of EGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-LC3
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsmRFPExpressionMammalianMutationFirst ATG is not included.PromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mNeonGreen
Plasmid#98876PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsert4xmts
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1/pcDNA3.1 ~ Human
Plasmid#140575PurposeExpression in mammallian cells or oocytesDepositorInsertCaV2.1 Human (CACNA1A Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SGEP
Plasmid#111170PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterSFFVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only