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pAT9675-CBE
Plasmid#163007Purposeplasmid expressing CBEDepositorInsertCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET11b_MutT
Plasmid#73155PurposeExpresses in E. coli, E coli MutTDepositorInsertMutT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDY1656 STITCHR Gluc AAVS1 payload
Plasmid#234827PurposeGluc exon 2 with 50bp AAVS1 homology armsDepositorInsertGluc exon 2
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-eGFP-hTau P301L-WPRE
Plasmid#233043PurposeTo Express a GFP human Tau P301L fusion protein from a CMV promoterDepositorInserteGFP-hTau P301L fusion (MAPT Synthetic, Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PS6K-mcherry-iATPsnFR1.1
Plasmid#227238PurposeInducible ATP sensor for E. coliDepositorInsertiATPsnFR1.1
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cre
Plasmid#198712PurposeFor lentiviral expression of Cre recombinase in mammalian cells.DepositorInsertPgk-Cre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterPgkAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Blast
Plasmid#227260PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1_Short_V5-GFP
Plasmid#221499PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterUBCAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140168PurposeExpresses a regulatable secretory cargo for Tetrahymena thermophilaDepositorInsertssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-D131Y
Plasmid#113424PurposeExpresses hA3A-BE3-D131Y in mammalian cellsDepositorInserthA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_D131YPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11_Ec_ORF209
Plasmid#69029PurposeExpresses E.coli ORF209/ADPRase in bacterial cellsDepositorInsertORF209/ADPRase
UseTags-ExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149198)
Plasmid#80248Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pKK-BiFC-Venus
Plasmid#105804PurposeConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.DepositorTypeEmpty backboneUseFlp-in competentTagsVN173 (I152L); VC155ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
Plasmid#140659PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(F74G) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TVAmCherry-2A-oG
Plasmid#104330PurposeAAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)DepositorInsertsTVA-mCherry fusion protein
Optimised Rabies Glycoprotein (oG)
UseAAV; Adeno associated viral vectorTagsExpressionMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLGB13
Plasmid#126618PurposeSuicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, GFP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pRSETB/mStayGold(c4)
Plasmid#212018PurposeFor N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.DepositorInsertmStayGold(c4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZT-C13-L1
Plasmid#62196PurposeChr.13/CLYBL TALEN expression vectorDepositorInsertLeft CLYBL TALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIP403
Plasmid#122028PurposePlasmid for expression of gene of interest in Lactobacillus plantarum and L. sakeiDepositorInsertgusA
UseTagsExpressionBacterialMutationPromotersppAAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-SOX2
Plasmid#47540PurposeTet inducible knockdown of SOX2.DepositorInsertSOX2 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
P4-PE
Plasmid#211375PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertP4-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV FKBP-V5-sTurboID-high affinity (N)
Plasmid#153004PurposeExpresses high-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (N) (G78)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
APEX2-NLS
Plasmid#124617Purposeproximity biotinylationDepositorInsertAPEX2-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab11a-7
Plasmid#56444PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab11a (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Plasmid#163056Purposemito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)DepositorInsertmito-roGFP2-Grx1
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HRE-luciferase
Plasmid#26731PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.DepositorInsertHypoxia Responsive Elements (3)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterEF1 alpha and TREAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbALFA
Plasmid#184678PurposeNIR-Fb to ALFA-tagDepositorInsertNIR-FbALFA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorInsertPark2 (PRKN Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralTagsExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0373
Plasmid#141594PurposeLentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFYA (NFYA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only
