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Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab7a-7
Plasmid#55127PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab7a (RAB7A Human)
UseTagsmCherryExpressionMammalianMutationN117S in NM_004637.5PromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2B
Plasmid#15781DepositorInsertUbiquitin conjugating enzyme UbE2B (UBE2B Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRTagsExpressionMammalianMutationDR1 A>TPromoterU6Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Rab5DN(S34N)
Plasmid#35139DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationDominant Negative- S34NPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise2-IL-1beta-mNeonGreen
Plasmid#166783PurposeFRET analysis (pro-IL-1beta cleavage) in living cellsDepositorInsertmTurquoise2-IL-1b-mNeongreen (IL1B Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-ApoE2
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApoE2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-CD3z
Plasmid#135993PurposeModular CD3z alone CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-OTUB1 WT-pcDNA3.1
Plasmid#118209PurposeExpresses FLAG-OTUB1 WT in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationnonePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TYK2
Plasmid#23908DepositorInsertTYK2 (TYK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Calnexin-N-14
Plasmid#55005PurposeLocalization: Endoplasmic Reticulum, Excitation: 587, Emission: 610DepositorInsertCalnexin (CANX Human)
UseTagsmCherryExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tubulin K40R
Plasmid#105303PurposeTo study effects of acetylation-null tubulinDepositorInsertalpha tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40RPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits