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pAP684-3

Plasmid

#99489

Purpose

TEV::eGFP::ER::Ollas::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::Ollas::linker::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCM1.52

Plasmid

#19592

Depositor

Geraldine Seydoux

Insert

lip-1 prom in PDONRP4P1R

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-BLMP-1-pcDNA3.1-

Plasmid

#52514

Purpose

expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

blmp-1 (blmp-1 Nematode)

Use

Tags

6xHis

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHZ054

Plasmid

#27505

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pmyo-3::yfp::lin-14_3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 10, 2011

Availability

Academic Institutions and Nonprofits only

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pCM5.64

Plasmid

#19372

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

puf-5 3'utr in pDONRP2RP3 (puf-5 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pGC236

Plasmid

#19675

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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oma-2 ORF in L4440

Plasmid

#82548

Purpose

oma-2 RNAi feeding construct

Depositor

Sean Ryder

Article

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

oma-2 ORF

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pNH030

Plasmid

#42149

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::2xNLS

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMALac-OMA-1(1-182)

Plasmid

#80350

Purpose

Expresses OMA-1 (1-182) in bacteria for protein purification purposes

Depositor

Sean Ryder

Article

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

OMA-1 (oma-1 Nematode)

Use

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Encodes AA residues 1-182

Promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKPY738

Plasmid

#72674

Purpose

C. elegans let-858 3' UTR (Gateway Entry Clone)

Depositor

David Tirrell

Insert

let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Use

Tags

MBP

Expression

Bacterial

Mutation

K196R

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pCM5.66

Plasmid

#19370

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

pos-1 3'utr in pDONRP2RP3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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U653-GFP

Plasmid

#24477

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEG587

Plasmid

#40196

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)

Use

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

S458A; exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge