Addgene

We narrowed to 17,054 results for: gateway

Showing: 9161 - 9180 of 17054 results

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pMetYC-Dest

Plasmid

#105081

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

Cub-PLV

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCWX-UBI-hTert-PGK-BSD

Plasmid

#114316

Purpose

Constitutive expression of the hTert protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-UCHL3

Plasmid

#22564

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL3 (UCHL3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP53

Plasmid

#22606

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP53 (USP53 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 65 aa

Promoter

CMV/TO

Available since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP20

Plasmid

#22573

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP20 (USP20 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAG413 GAL ORF1 Shuffle1 NLS

Plasmid

#145794

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Payero et al Journal of the South Carolina Academy of

Insert

ORF1 Shuffle1 NLS

Use

Tags

Expression

Yeast

Mutation

Enhanced Nucleur Localization Signal

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM9

Plasmid

#222565

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PRDM9 (PRDM9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP39

Plasmid

#22581

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP39 (USP39 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
Flag-HA-USP15

Plasmid

#22570

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP15 (USP15 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly ZER1

Plasmid

#191540

Purpose

Protein expression of ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

ZER1 (ZER1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP13

Plasmid

#22568

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP13 (USP13 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR U6-hTERTsh

Plasmid

#111385

Purpose

Knock down of hTERT transcript

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

shRNA C3 against hTERT

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Active Wnt16-V5

Plasmid

#43825

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

Wnt16 (WNT16 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP14

Plasmid

#22569

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP14 (USP14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_ECFP

Plasmid

#118857

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

ECFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX305_3xFLAG_RELA1

Plasmid

#220324

Purpose

Expresses full length human RELA with an N-terminal 3xFLAG tag

Depositor

Jonathan Brown

Insert

RELA (RELA Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

hPGK

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Active Wnt7A-V5

Plasmid

#43816

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7A (WNT7A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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