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pMAL-p2x-LanCL2

Plasmid

#154192

Purpose

To express MBP-LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Maltose binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00007

Plasmid

#166725

Purpose

non targeting sgRNA

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

Non-targeting guide

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-acta2

Plasmid

#78686

Purpose

acta2 promoter for Gateway cloning

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-GATA2

Plasmid

#125028

Purpose

doxycycline-inducible expression of human GATA2 in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dL5-2XG4S-mCer3

Plasmid

#73207

Purpose

Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

KozATG-dL5-2XG4S-mCer3

Use

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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Integrin beta5-2XEGFP

Plasmid

#139779

Purpose

Detecting Integrin beta5 by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

Insert

Integrin subunit beta5 (ITGB5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Use

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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RIP7-RLuc-YFP-pBS

Plasmid

#72482

Purpose

Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

Depositor

Vincent Poitout

Article

Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

Inserts

rat ins2 gene promoter (Ins2 Rat)
luciferase
eYFP

Use

Luciferase

Tags

eYFP and renilla luciferase

Expression

Mammalian

Mutation

eYFP varient

Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pPN186

Plasmid

#91573

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN187

Plasmid

#91574

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK7-EE-NLS hygro

Plasmid

#87780

Purpose

Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7 (Map2k7 Mouse)

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
peGFP C3-SPG20 8xV

Plasmid

#218579

Purpose

Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

changed Tryptophan 419, Leucine 438, Isoleucine 4…

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_G671R/N865K

Plasmid

#204478

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B G671R/N865K mutants

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

G671R / N865K

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Gfp-rt-vasa

Plasmid

#167322

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

rt vasa 3'UTRs (ddx4 rainbow trout)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-ECT2

Plasmid

#183835

Purpose

Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 homology arms with mNeonGreen-linker (ECT2 Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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