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pET-22b(+)_PelB-BN009K-H-FLAG-His

Plasmid

#218049

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN009K-H

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN00AZ-W-FLAG-His

Plasmid

#218039

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN00AZ-W

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN00H3-S-FLAG-His

Plasmid

#218038

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN00H3-S

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN00FH-2-FLAG-His

Plasmid

#218037

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN00FH-2

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN00B0-E-FLAG-His

Plasmid

#218035

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN00B0-E

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-E993A

Plasmid

#203698

Purpose

expresses ATP10B with the pathogenic mutation E993A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

E993A

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-E210A

Plasmid

#203697

Purpose

expresses ATP10B with the catalytic mutation E210A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

E210A

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-D433N

Plasmid

#203696

Purpose

expresses ATP10B with the catalytic mutation D433N

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

D433N

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-V748L

Plasmid

#203702

Purpose

expresses ATP10B with the pathogenic mutation V748L

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

V748L

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-I1038T

Plasmid

#203700

Purpose

expresses ATP10B with the pathogenic mutation I1038T

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

I1038T

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-R153X

Plasmid

#203701

Purpose

expresses ATP10B with the pathogenic mutation R153X

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

R153X

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mOct4-POU

Plasmid

#206393

Purpose

Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

Oct4 (Pou5f1 Mouse)

Use

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/GFP4_Seq1.3

Plasmid

#206136

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26G45E-IRES-GCaMP6s

Plasmid

#188237

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

p.G45E

Promoter

Tight TRE promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

Plasmid

#204426

Purpose

Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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