pET28 mOct4-POU
(Plasmid #206393)

• Purpose: Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification
• Depositing Lab: Ralf Jauch
• Publication: Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 10.1093/molbev/msab075.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Oct4
• Species: M. musculus (mouse)
• Mutation: POU DNA binding domain only
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry