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Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only
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dCas13d-BFP
Plasmid#215720PurposeCatalytically inactive dCasRx with 2A-BFPDepositorInsertdCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
UseTagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallin and cmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_Keima
Plasmid#127140DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianMutationPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in PET28a for protein expression
Plasmid#39297DepositorInsertEB1 (MAPRE1 Human)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
L1-neo-TET
Plasmid#51284Purposeexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassetteDepositorInsertL1RE1 (L1RE1 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1beta
Plasmid#17651DepositorInsertHP1 beta (CBX1 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47 PINK1 C-GFP
Plasmid#13316DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-624-919
Plasmid#104200PurposeExpresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion proteinDepositorInserthuman androgen receptor aa 624-919 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dynamin 2 K44A
Plasmid#22301DepositorInsertDynamin 2 K44A (Dnm2 Rat)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRetrosuper Myc shRNA
Plasmid#15662DepositorInsertMyc shRNA (MYC Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG_mRuby2_smFP FLAG
Plasmid#59760Purposeencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-ER-5
Plasmid#55294PurposeLocalization: Endoplasmic Reticulum, Excitation: 399, Emission: 456DepositorInsertER (CALR Human)
UseTagsmTagBFP2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pINDUCER13 miR-200c-141
Plasmid#81020PurposeLentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.DepositorInsertmiR-200c (MIR200C Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GpNLuc
Plasmid#208840PurposeExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only