GST-AR-624-919
(Plasmid
#104200)
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PurposeExpresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104200 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-5x-1
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 5800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL-1 blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman androgen receptor aa 624-919
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SpeciesH. sapiens (human)
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Insert Size (bp)890
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GenBank IDAAA51886.1
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer don't have
- 3′ sequencing primer don't have (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-AR-624-919 was a gift from Elizabeth Wilson (Addgene plasmid # 104200 ; http://n2t.net/addgene:104200 ; RRID:Addgene_104200) -
For your References section:
Electrostatic modulation in steroid receptor recruitment of LXXLL and FXXLF motifs. He B, Wilson EM. Mol Cell Biol. 2003 Mar;23(6):2135-50. PubMed 12612084