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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al ChemBioChem, 8 March 2024

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mustard Weed, Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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VF-Sensor

Plasmid

#208927

Purpose

in vitro RSK-Sensor for luminescent detection of RSK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

SOS1 (SOS1 Human)

Use

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PDE4B-Sensor

Plasmid

#208929

Purpose

in vitro JNK-Sensor for luminescent detection of JNK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)

Use

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-EGFP

Plasmid

#210378

Purpose

Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP4 (ORF1ab SARS-CoV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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NSP2-Flag

Plasmid

#210344

Purpose

Expresses SARS-CoV-2 NSP2 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Drosophila Usp46-V5

Plasmid

#217620

Purpose

Mammalian expression of V5 tagged Drosophila Usp46

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

USP46 (Usp12-46 Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Human USP46

Plasmid

#217615

Purpose

Mammalian expression of USP46

Depositor

Ethan Lee

Article

Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

Insert

USP46 (USP46 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2

Plasmid

#204976

Purpose

Co-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tag

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Use

Tags

ALFA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-6xHp

Plasmid

#214416

Purpose

Lipid droplet localization of CFAST fused to Halotag-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

deletion

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-MAFG-V5-miniTurbo

Plasmid

#207818

Purpose

C-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

MAFG-V5-miniTurbo

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-PermE

Plasmid

#209446

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from ermE* promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(HA)

Plasmid

#195022

Purpose

Gateway vector containing HA-tagged Creb5

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-bla

Plasmid

#189991

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 E2E1- Prg4 promoter-luciferase

Plasmid

#195035

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.10 E3E2E1-Prg4 promoter-luciferase

Plasmid

#195036

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

Plasmid

#195037

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge