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CL7_2xNLS_iGeoCas9(G)_2xNLS_His

Plasmid

#222989

Purpose

Bacterial expression of iGeoCas9(G) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(G)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH145_mCherry-YIPF4 LIR mut

Plasmid

#214973

Purpose

mCherry tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mCherry-YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK1-CXCR4-MM4

Plasmid

#215846

Purpose

Expression of CXCR4 target with mismatch (MM) 4_

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

CXCR4 target with mismatch (MM) 4x

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q443P

Plasmid

#216402

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 443 to Proli…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge