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Plasmid#19731DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only
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pcDNA-Cas9-T2A-STOP-TdT
Plasmid#126425PurposeExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)DepositorInsertCas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationalanine-6 of TdT has been changed to a STOP codonPromoterpCMVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
zw3 (shaggy) pbOVI
Plasmid#38749DepositorInsertzw3 (sgg Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
anti-GFP scFv [N86/38] with sortase tag
Plasmid#204419PurposeMammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.DepositorInsertRecombinant mouse scFv targeting GFP (Aequorea victoria)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000072225
Plasmid#78160PurposeshLacZ controlDepositorInsertLacZ
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆bund
Plasmid#74889Purposemammalian expression of the bundling activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationL757A, K759APromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q-R388G
Plasmid#184251PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000072254
Plasmid#78161PurposeLuciferase controlDepositorInsertLuciferase
UseRNAiTagsExpressionMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ENL)
Plasmid#69212PurposeLentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])DepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1ecto&tmd-BTLAicd-mGFP
Plasmid#180794PurposeExpression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mGFPDepositorUseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGE1.0
Plasmid#165986PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
spectinomycin/streptomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
R-Q111R-N122D
Plasmid#167178PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-R-Q111R-N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged R at 111 to Q and changed D at 122 to NPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000231725
Plasmid#78163PurposeshRFP controlDepositorInsertRFP
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 W201E
Plasmid#186471PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationW201E (no more binding to lipid droplets)PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA
Plasmid#154780Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertCRFR1 (Crhr1 Rat)
UseTagsHAExpressionMammalianMutation95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP
Plasmid#47905PurposeAn AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.DepositorInserthChR2(H134R)
UseAAVTagsiRFPExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAav-MDM2-PQS2-3xHA
Plasmid#84886PurposerAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassetteDepositorUseAAVTagsPQS2 3xHAExpressionMutationPromoternoAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-Yap S127A
Plasmid#46050DepositorInsertYes-kinase associated protein (YAP1 Human)
UseEntry vectorTags2X Flag tagExpressionMutationS127APromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-CD79B-YFP
Plasmid#187006PurposeExpression of human CD79B as YFP fusion proteinDepositorInsertCD79B (CD79B Human)
UseLentiviralTagsYFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only