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Plasmid#210469PurposeDonor plasmid for knock-in GFP-CD19 into the human INS locusDepositorInsertINS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
UseTags6xHis-CUboExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W
Plasmid#200475PurposeLentiviral vector expressing gRNA targeting GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID-GFP
Plasmid#209405PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-mTP-TurboID-GFP
Plasmid#209404PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in plantaDepositorInsertmTP-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-TurboID-3XNLS
Plasmid#209400PurposeTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TGA-rt9us-antiGFP
Plasmid#206954PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorInsertRPA3 (RPA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD1
Plasmid#210264PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD1PromoterCMV/TetO2Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD2
Plasmid#210263PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD2PromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-LAMP1
Plasmid#207788PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus for lysosome visualization.To be co-transfected with sgRNA plasmid px330-PITCh-LAMP1 Addgene #207787DepositorArticleInsertLAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hy_U5:34A eGFP SV40
Plasmid#195064PurposeExpresses eGFP when there is readthrough transcription from the Drosophila U5:34A geneDepositorInsertsnRNA:U5:34A (snRNA:U5:34A Fly)
UseTagsExpressionInsectMutationPromoterU5:34AAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID-GFP
Plasmid#209389PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l2-A'-IRES-EmGFP
Plasmid#209125PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFPDepositorInsertSUMO-A'-l2-A'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only