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Showing: 8981 - 9000 of 12980 results

  1. pLKO_FLAG.HA.C_NGFR

    Plasmid
    #158257
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.HA.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    158257-plasmid-map-sequence-id-329535

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  2. pLKO_FLAG.NWS.C_NGFR

    Plasmid
    #158258
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.NWS.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    158258-plasmid-map-sequence-id-327860

    Enlarge

  3. pLKO_FLAG.V5.C_NGFR

    Plasmid
    #158259
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.V5.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    158259-plasmid-map-sequence-id-327882

    Enlarge

  4. pLKO_FLAG.VSVg.C_NGFR

    Plasmid
    #158260
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.VSVg.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    158260-plasmid-map-sequence-id-327912

    Enlarge

  5. pLKO_HA.NWS.C_NGFR

    Plasmid
    #158261
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    158261-plasmid-map-sequence-id-327913

    Enlarge

  6. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

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  7. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

    Enlarge

  8. pYPQ284-RVRR

    Plasmid
    #160327
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a RVRR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N563R, K569V, N573R and K625R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    160327-plasmid-map-sequence-id-317695

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  18. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

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Showing: 8981 - 9000 of 12980 results