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Plasmid#194415PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRproDepositorInsertmTurquoise2 CDS
UseSynthetic Biology; Used as donor dna in genome in…TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPERpuro-BglII-HsXRN1-t3_V
Plasmid#147797PurposeMammalian Expression of HsXRN1-t3-shRNADepositorInsertHsXRN1-t3-shRNA (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL752-hGH-polyA-PGK-puroR
Plasmid#191682PurposeDonor plasmid backbone PGK-puroRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GFP-rG1
Plasmid#188967PurposeIPTG inducible GFP with sgRNADepositorInsertsGFP
sgRNA: agtggaaaacaatgcgaccgactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GFP-rG2
Plasmid#188968PurposeIPTG inducible GFP with sgRNADepositorInsertsGFP
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA5460
Plasmid#184855PurposeEncodes G418 resistance and PhiC31-excisable hTFAM geneDepositorInsertTFAM (TFAM Human)
UseRetroviral; Phic31 attp-attb recombination/excisi…TagsExpressionMammalianMutationWTPromoterLTRAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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KBB106
Plasmid#185065PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domainDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal region, truncated so C-terminal h…PromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Soat2_Luc
Plasmid#177623PurposeCloned human Soat2 promoter containing exon1 and 2 coupled to luciferaseDepositorInserthuman Soat2
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 AAAA
Plasmid#163579Purposeexpress Dcp2 300 AAAA at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); mutati…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 ΔH1
Plasmid#163578Purposeexpress Dcp2 300 ΔH1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); Lysine…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK6_011
Plasmid#123865PurposeEncodes the inverted attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK systemDepositorInsertattB (phiC31) inverted
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_012
Plasmid#123866PurposeEncodes the inverted attB BxBI transposon recognition site cassette as a type 6 part to be used in the MTK systemDepositorInsertattB (BxBI) inverted
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; F4A)
Plasmid#110757PurposeBacterial expression of Ubiquitin (1-76; F4A)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationF4APromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only