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p663-UBC-miniTurbo-V5-DYRK4_IDG-K

Plasmid

#158184

Purpose

Gateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-DYRK4 (DYRK4 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDKL2_IDG-K

Plasmid

#158181

Purpose

Gateway destination clone of CDKL2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDKL2 (CDKL2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDKL1_IDG-K

Plasmid

#158176

Purpose

Gateway destination clone of CDKL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDKL1 (CDKL1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CAMK1G_IDG-K

Plasmid

#158168

Purpose

Gateway destination clone of CAMK1G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CAMK1G (CAMK1G Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-BRSK2-V5-miniTurbo_IDG-K

Plasmid

#135252

Purpose

Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BRSK2-V5-miniTurbo (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-BRSK2_IDG-K

Plasmid

#135271

Purpose

Gateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-BRSK2 (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-BRSK2-V5_IDG-K

Plasmid

#135254

Purpose

Gateway destination clone of BRSK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BRSK2-V5 (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHL-avitag3

Plasmid

#99847

Purpose

mammalian expression with secretive signal and avi tag for biotinylation

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Use

Tags

Avi

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Use

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK11B-V5-miniTurbo_IDG-K

Plasmid

#189914

Purpose

Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK11B (CDK11B Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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CD200RCD4d3+4-bio-linker-His

Plasmid

#36153

Purpose

A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200R (Cd200r1 Rat)

Use

Tags

His tag, biotinylation peptide, and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK13_IDG-K

Plasmid

#135266

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-CDK13_IDG-K

Plasmid

#135267

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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GlycophorinE-CD4d3+4-bio

Plasmid

#73095

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin E (GYPE Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDKL5_IDG-K

Plasmid

#170578

Purpose

Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDKL5 (CDKL5 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

E854G- please see depositor comments

Promoter

Ubiquitin

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK19_IDG-K

Plasmid

#158175

Purpose

Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK19 (CDK19 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK7_IDG-K

Plasmid

#158194

Purpose

Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK7 (CDK7 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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