unc5bCD4d3+4-bio-linker-His
(Plasmid
#36150)
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PurposeEXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-bio-linker-His tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7760
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameunc5b
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Alt nameunc5b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1150
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MutationL2V
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Entrez Geneunc5b (a.k.a. unc5h2)
- Promoter CMV
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Tags
/ Fusion Proteins
- ratCD4d3+4 (C terminal on backbone)
- biotinylation peptide (C terminal on backbone)
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
unc5bCD4d3+4-bio-linker-His was a gift from Gavin Wright (Addgene plasmid # 36150 ; http://n2t.net/addgene:36150 ; RRID:Addgene_36150) -
For your References section:
A cell surface interaction network of neural leucine-rich repeat receptors. Sollner C, Wright GJ. Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18. 10.1186/gb-2009-10-9-r99 PubMed 19765300