Addgene

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Prrx1:TFPnls-T2a-Cre-Ert

Plasmid

#111150

Purpose

Limb connective tissue specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-Cre-Ert

Use

Cre/Lox

Tags

Expression

Mutation

wt

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53

Plasmid

#196267

Purpose

Mammalian expression of 3xHA tagged wild-type p53

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 (Trp53 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mATF4

Plasmid

#24874

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mouse ATF4 (Atf4 Mouse)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-MyoD

Plasmid

#8398

Depositor

Andrew Lassar

Article

Skapek et al Mol Cell Biol 1996 Dec;16(12):7043-53.

Insert

MyoD (Myod1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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Plxna1-Fc-His

Plasmid

#72122

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

Lac

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Use

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Article

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pMIH-TOMM20-Halo

Plasmid

#111626

Purpose

Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TOMM20 (Tomm20 Mouse)

Use

Retroviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-mSmo

Plasmid

#25395

Depositor

Philip Beachy

Article

Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Insert

mouse Smoothened cDNA (Smo Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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W45A mutant of H3K9me3 biosensor

Plasmid

#120808

Purpose

FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Mortalin/GRP75 [N52A/42.1R]

Plasmid

#114548

Purpose

Mammalian Expression Plasmid of anti-Mortalin/GRP75 (Mouse). Derived from hybridoma N52A/42.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mortalin/GRP75 (Mus musculus) recombinant mouse monoclonal antibody (Hspa9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG SREBP2

Plasmid

#32018

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pKT-mPDGFb-IRES-Katushka

Plasmid

#133082

Purpose

Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

PDGFb (Pdgfb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGG-NFIB2

Plasmid

#112700

Purpose

Expression of NFIB2 protein and GFP

Depositor

Richard Gronostajski

Insert

Nuclear Factor I B2 (Nfib Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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His-tagged FL Rec8

Plasmid

#45243

Purpose

raise antibody against Rec8

Depositor

Michael Lampson

Article

Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

Insert

Rec8 (Rec8 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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