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Plasmid#209763PurposeExpression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoterDepositorInsertuMASS_LF
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-uMASS_1
Plasmid#209762PurposeExpression of uMASS_1 in mammalian cells under CAG promoterDepositorInsertuMASS_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG-F6C
Plasmid#176546PurposeExpresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange phenylalanine 6 to cysteine; change glutam…PromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_007
Plasmid#123760PurposeEncodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK systemDepositorInsertPTS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-AKT-PH
Plasmid#67674PurposeGateway entry clone containing AKT-PH with no stop codonDepositorInsertAKT-PH
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-FL-TagBFP
Plasmid#120921PurposeExpresses BFP tagged Ptch1DepositorInsertptch1 (Ptch1 Mouse)
UseTagsTagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (K999R)
Plasmid#170142PurposeExpresses CHD7 (K999R) in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationK999RPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
UseTags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only