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Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.Myc.p44
Plasmid#39220DepositorInsertp44 (GTF2H2 )
UseTagsMycExpressionInsectMutationPromoterPp10Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP
Plasmid#174893Purposeamber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassetteDepositorInsertmCherry and EGFP
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT-FLAG
Plasmid#31385DepositorTypeEmpty backboneUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pLS-CG
Plasmid#12161DepositorInsertCMV-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-p50
Plasmid#107977PurposeExpresssion of NF-kB p50DepositorInsertNF-kB p50 (Nfkb1 Mouse)
UseTagsN/AExpressionMammalianMutationAmino acids VQACI added to C-terminusPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459-puro-hPKAalpha
Plasmid#185379PurposeFor mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alphaDepositorInsertPRKACA (PRKACA Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-G418
Plasmid#158550PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBABE.is4-mOpn
Plasmid#74410PurposeExpresses mouse Osteopontin from transcript tv4 in mammalian cellsDepositorInsertmouse Opn (Spp1 Mouse)
UseTagsnoneExpressionMammalianMutationPromoterMMLVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]
Plasmid#86453PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)DepositorInsert3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SaCas9
Plasmid#107032PurposeAAV plasmid expressing SaCas9 under control of miniCMV promoterDepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-S320A
Plasmid#112491PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationS320APromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMMBGent
Plasmid#45475DepositorInsertGentamycin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TMC1-TwinStrepII
Plasmid#83267PurposeExpression of tagged protein in mammalian cellsDepositorInsertTMC1 (Tmc1 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-hPPM1B
Plasmid#185383PurposeFor mammalian expression of shRNA: GAGCAGAAGAGGATGAATTTA that targets human PPM1BDepositorInsertPPM1B (PPM1B Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only