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Plasmid#121957PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertBsCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AmsgRNA
Plasmid#121956PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAmCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
U_(+36GFP)-Cre.ape
Plasmid#71749PurposeU+36GFP-Cre fusion expression in E. ColiDepositorInsertU_(pos36GFP)-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nirButterfly
Plasmid#136590PurposeExpression of a genetically-encoded voltage indicator nirButterflyDepositorInsertnirButterfly
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_arr2
Plasmid#149639Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA3 (pMT2)
Plasmid#75185PurposeMammalian expression of ATP2A3DepositorInsertATP2A3 (Atp2a3 Rat)
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-N-Flag-8His
Plasmid#157677PurposeN-terminal tagging vectorDepositorInsertFlag-8His
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-PGK-SNAP-MDMUT-SOX2
Plasmid#115688PurposeConstitutive expression of a SOX2 protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for controlDepositorInsertSNAP-MDMUT-SOX2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-BirA
Plasmid#71752PurposeAurein1.2+36GFP-BirA fusion for E.Coli expressionDepositorInsertAurein1.2+36GFP-BirA
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G_arr2
Plasmid#149571Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only