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Plasmid#190831PurposeFor mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
hE-cadherin/Δp120
Plasmid#45770DepositorInserthE-cadherin/Δp120 (CDH1 Human)
UseTagsExpressionMammalianMutationAmino acids 762-764 converted from EED to AAA to …PromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
GB-Dcp1b
Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorInsertmRNA decapping enzyme 1b (DCP1B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertNDM-1 (blaNDM-1 Klebsiella pneumoniae)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046757
Plasmid#145657PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSP3-FR-2Strep
Plasmid#210315PurposeExpresses SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertNSP3 (ORF1ab SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
REEP6-EGFP
Plasmid#210364PurposeExpresses REEP6 fused with EGFP in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mH2A1.1-CT-MYC
Plasmid#45166DepositorInsertH2Afy (MACROH2A1 Human)
UseTagsHIS and MYCExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Calnexin-HT-mGold
Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDA093
Plasmid#131390Purposegeneration of Tet-On RPEDepositorInsertORFeus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin5
Plasmid#41916DepositorInsertCUL5 (CUL5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEY11
Plasmid#191017PurposeFluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertUltrapanneuronal
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin1
Plasmid#41911DepositorInsertCUL1 (CUL1 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
UseTagsHis-tag and Mitochondria localization sequenceExpressionMammalianMutationPromoterCMV, SP6Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only