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Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ĪPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
(GGS)9_(+36GFP)-Cre.ape
Plasmid#71747Purpose+36-GFP-Cre fusion expression in E.ColiDepositorInsert(GGS)9_+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-AkCas12b
Plasmid#121950PurposeE. coli expression, protein purificationDepositorInsertAkCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tagDepositorInsertS-Luc_CLIP
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resiā¦PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only