Addgene

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pEGFP-NUP188

Plasmid

#159893

Purpose

Expresses NUP188 in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

Insert

NUP188 (NUP188 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

To avoid NotI site, first three amino acids are d…

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1deltaART

Plasmid

#174804

Purpose

Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1deltaART (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 785-1014

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1

Plasmid

#173933

Purpose

Bacterial expression of PARP1 lacking Zn1 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2

Plasmid

#173935

Purpose

Bacterial expression of PARP1 lacking Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 (V762A)

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1543

Plasmid

#205963

Purpose

NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH Cascade with His14-tagged Csf3

Use

Tags

14xHis on Csf3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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FMR1-E17C-3xFLAG

Plasmid

#157784

Purpose

donor plasmid for C-terminal FLAG tag fusion of human FMR1

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1-FLAG (FMR1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gDNMT1.Cas9-T2A-GFP

Plasmid

#170362

Purpose

Plasmid used for Crispr/cas9 based disruption of human DNMT1.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

Plasmid

#198035

Purpose

Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

SINTBAD (TBKBP1 Human)

Use

Tags

EGFP and GST-TEV

Expression

Insect

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Use

Tags

pHiuorin

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-SspB-HaloTag-p63DH

Plasmid

#176129

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-U6sgbbBsmbI-puro-2A-Fluc

Plasmid

#100277

Purpose

Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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ins:MCS; cryaa:RFP

Plasmid

#110282

Purpose

A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

insulin

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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