Showing: 861 - 880 of 1088 results
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Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
UseTagsFLAGExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS Y125A
Plasmid#154769Purposeplasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
UseTagsFLAGExpressionMammalianMutationY125APromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA
Plasmid#154775Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAA in GFP reporter, UUA anticodon and G10A, …PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG
Plasmid#154777Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAA 150TAG in GFP reporter, hybrid PylT with …PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
PB-TetR-tdTomato
Plasmid#212958PurposepiggyBac donor vector containing TetR-tdTomato for random integration by PBaseDepositorInsertTetR-tdTomato
UseMouse Targeting; Piggybase integration (transposo…TagstdTomatoExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-LacI-eGFP
Plasmid#213021PurposepiggyBac donor vector containing LacI-eGFP for random integration by PBaseDepositorInsertLacI**-eGFP
UseMouse Targeting; Piggybase integration (transposo…TagseGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast
Plasmid#205540PurposeExpress HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance markerDepositorInsertGag-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterTRE3GSAvailabilityAcademic Institutions and Nonprofits only -
pJAM1.19_PB-CIT-PGK-Blast
Plasmid#205564PurposeExpress human CITDepositorInsertCIT (CIT Human)
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG3-tdT
Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-NLS-tdiRFP670-2A-LBR
Plasmid#82620PurposeExpresses NLS-tdiRFP-2A-LBR in mammalian cellsDepositorInsertNLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorInsertEsrrb-IRES-Venus (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailabilityAcademic Institutions and Nonprofits only -
KA2880_pPB-TREtight-FLAG-Esrrb-InO
Plasmid#124178PurposepiggyBac-based Dox-inducible expression of FLAG-EsrrbDepositorInsertFLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailabilityAcademic Institutions and Nonprofits only -
KA2944_pPB-TREtight-Esrrb-FLAG-InO
Plasmid#124179PurposepiggyBac-based Dox-inducible expression of Esrrb-FLAGDepositorInsertEsrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC/LLAA
Plasmid#124399PurposeFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624) and L897A/…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 861 - 880 of 1088 results