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Plasmid#69287PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only
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pCMV-PRLSS-SH2-KDEL
Plasmid#209716PurposeMammalian expression of SH2 localized to endoplasmic reticulumDepositorInsertPRLSS-SH2-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC6-P1A6
Plasmid#202847PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6DepositorInsertEGFP/BC6
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU51
Plasmid#167677PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6_(Gluc)_INT(GenPurpClon)
Plasmid#68433PurposeGeneral Purpose cloning vector for "INT"-like constructs under U6-promoter expression.DepositorInsertINT construct
UseCRISPR; Cloning vector for generating int-like co…TagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p32Fn
Plasmid#158153PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-boxB-MS2
Plasmid#75396PurposesgRNA1-boxB-MS2DepositorInsertsgRNA1-boxB-MS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pActinin-miRFP670nano
Plasmid#127432PurposeAlpha actinin labeling with near-infrared fluorescent proteinDepositorInsertAlpha-actinin-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_9xD3B_Cluc_Norm
Plasmid#68414PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.DepositorInsertCypridina Luciferase-2A-Venus YFP
UseLentiviral and LuciferaseTagsExpressionMutationYFP: M153T,V163A,S175G ("Venus") YFP Va…PromoterminimalCMVAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D
Plasmid#69289PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_LamB1
Plasmid#181999PurposeCMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell linesDepositorInsertSTf-LamB1
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x6
Plasmid#58776PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x4
Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorInsertCrowdedness-sensitive linker domain
UseTagsmClover2 and mRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP
Plasmid#175522PurposeNuclear expression of HaloTag11 in mammalian cellsDepositorInsertH2B-HaloTag11
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TB:4xHRE_RE:MiniP
Plasmid#178313Purpose4 copies of the HRE DNA binding motifDepositorInsert4 copies of the heat shock DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)
Plasmid#163063PurposeEntry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)DepositorInsertCheRiff
UseGateway entry vectorTagseGFPExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only