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Addgene
Showing: 861 - 880 of 34733 results
  1. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to Ī±-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Insert
    csgBACEFG, with Ī±-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression systemā€¦
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. M13-kmR

    Plasmid
    #206861
    Purpose
    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
    Depositor
    Insert
    Kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pL0_AC-CEN_ARS_HIS

    Plasmid
    #202129
    Purpose
    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.
    Depositor
    Insert
    CEN_ARS_HIS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ARS sequence mutated to remove sapI site for domeā€¦
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pL0_EF-H4

    Plasmid
    #202178
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    H4 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pL0_EF-fcpA

    Plasmid
    #202164
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    fcpA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pL0_AC-H4

    Plasmid
    #202122
    Purpose
    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Insert
    H4 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 861 - 880 of 34733 results