Showing: 861 - 880 of 1186 results
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Plasmid#63792PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection markerDepositorInserteCFP-Twin-tag followed by URA3
UseE. coli cloning vectorTagseCFP-Twin-tagExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pJPN0018
Plasmid#112083PurposeeCFP module CTDepositorInserteCFP
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJPN0017
Plasmid#112082PurposeeCFP module CDS1nsDepositorInserteCFP without stop codon
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailabilityAcademic Institutions and Nonprofits only -
C9m
Plasmid#120958PurposeMoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertdeCFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCUU
Plasmid#159747PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-beta-1 in pcDNAI/Amp
Plasmid#55707PurposeAn amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.DepositorInsertmCer(1-158)-beta 1 (GNB1 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-beta-5 in pcDNAI/Amp
Plasmid#55708PurposeAn amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertmCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-5 was amplified via PCR, which added an N-t…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
Plasmid#114078PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)
Plasmid#114091PurposeCAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a (E174R/S542R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R and S542RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)
Plasmid#114092PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a (S542R/K548V/N552R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationS542R, K548V and N552RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)
Plasmid#114094PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (S542R/K607R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationS542R and K607RPromoterAvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs
Plasmid#17860DepositorInsertFLIPrbs
UseTagsECFP and EYFPExpressionBacterialMutationD208GPromoterAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs T135A
Plasmid#17861DepositorInsertFLIPrbs T135A
UseTagsECFP and EYFPExpressionBacterialMutationT135APromoterAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs Q235A
Plasmid#17862DepositorInsertFLIPrbs Q235A
UseTagsECFP and EYFPExpressionBacterialMutationQ235APromoterAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs F15A
Plasmid#17863DepositorInsertFLIPrbs F15A
UseTagsECFP and EYFPExpressionBacterialMutationF15APromoterAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs F16A
Plasmid#17864DepositorInsertFLIPrbs F16A
UseTagsECFP and EYFPExpressionBacterialMutationF16APromoterAvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs R141A
Plasmid#17865DepositorInsertFLIPrbs R141A
UseTagsECFP and EYFPExpressionBacterialMutationR141APromoterAvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 861 - 880 of 1186 results