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Plasmid#134282PurposeExpresses Flag-tagged GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v1
Plasmid#134281PurposeExpresses Flag-tagged GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v2
Plasmid#134280PurposeExpresses GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v1
Plasmid#134279PurposeExpresses GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK_v2
Plasmid#134271PurposeLentivector encoding Flag-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk isoform 2; 553 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-3HA-GK5_v2
Plasmid#134269PurposeLentivector encoding 3XHA-tagged GK5 (isoform 2)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTags3X HAExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-3HA-GK5_v1
Plasmid#134268PurposeLentivector encoding 3XHA-tagged GK5 (isoform 1)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTags3X HAExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5_v2
Plasmid#134260PurposeLentivector encoding Flag-tagged GK5 (isoform 2)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5_v1
Plasmid#134257PurposeLentivector encoding Flag-tagged GK5 (isoform 1)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-DNMT3L [N117/9.5R]
Plasmid#114514PurposeMammalian Expression Plasmid of anti-DNMT3L (Human). Derived from hybridoma N117/9.5.DepositorInsertanti-DNMT3L (Homo sapiens) recombinant mouse monoclonal antibody (DNMT3L Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-TRIP8b (exon 1a/5) [N291C/22R]
Plasmid#114563PurposeMammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.DepositorInsertanti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSico shMAP3K2 mouse
Plasmid#85657Purposeexpression of shRNA targeting mouse MAP3K2DepositorInsertshRNA targeting 3'UTR of Map3k2 (Map3k2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSico shMAP3K2 human
Plasmid#85656Purposeexpression of shRNA targeting human MAP3K2DepositorInsertshRNA targeting 3'UTR of MAP3K2 (MAP3K2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158)-RGS7t
Plasmid#55761PurposeAn amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertRGS7(202-477)-CFP(1-158) (RGS7 Human, Aequorea victoria)
UseTagsCFP(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationThe RGS sequence amino terminal to the GGL domain…PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucA
Plasmid#174047PurposeExpresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucA
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationALG8 alanine 506 to threoninePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucL
Plasmid#174048PurposeExpresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucL
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationLAMA4 glycine 1254 to valinePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only