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Plasmid#73510PurposeGFPo_PH pCFJ150 - contains C. elegans sequence optimized GFPDepositorInsertpmex-5::GFPo::PH::tbb-2
UseTagsGFPExpressionMutationPromotermex-5Available sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-WASP-GBD
Plasmid#30113DepositorUseTagsGSTExpressionBacterialMutationContains the Cdc42-interacting domain of WASP (aa…PromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sox1/2/3-2373/+3>nls::Cas9::nls
Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcB-His-CD)
Plasmid#176194Purposeexpresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
UseTagsGfcB-6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h2
Plasmid#86691PurposeFor transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
UseTagsHAExpressionMutationPromoterT3Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only