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Plasmid#148492PurposeMammalian Expression of HsYTHDC2_1069-1430DepositorInsertHsYTHDC2_1069-1430 (YTHDC2 Human)
UseTagsExpressionMammalianMutationone non silent mutation L1409Q annotated as natur…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1070-Ai211 (TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)
Plasmid#181764Purposetargeting vectorDepositorInsertKv2.1-tagged ChrimsonR-tdTomato and tTA2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-Dmp68_B
Plasmid#145966PurposeBacterial Expression of Dmp68DepositorInsertDmp68 (Rm62 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pPOU5F1-donor
Plasmid#176367PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPOU5F1 (POU5F1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRERF1-donor
Plasmid#176366PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTRERF1 (TRERF1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR2E3-donor
Plasmid#176369PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertNR2E3 (NR2E3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRORB-donor
Plasmid#176354PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRORB (RORB Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZBED9-donor
Plasmid#176344PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZBED9 (ZBED9 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSC22D3-donor
Plasmid#176355PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTSC22D3 (TSC22D3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-SspB-HaloTag-TRIO-DHPH1
Plasmid#176133PurposeHeterodimerization with iLID, Rac1 and RhoG activationDepositorInsertTRIO (TRIO Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorInsertWhi5-sg106 (WHI5 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3459)
Plasmid#160639PurposepEGB3a2 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(2)
Plasmid#163428PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT-CAmut
Plasmid#155250Purposefor mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
UseTagsExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19ER-CAmut
Plasmid#155249Purposefor mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (ER isoform) CA mutant (Usp19 Rat)
UseTagsExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF302-donor
Plasmid#132513PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF302 (ZNF302 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
YARS2_pNIC-CTB10H
Plasmid#153060PurposeExpression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2DepositorInsertYARS2 (YARS2 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c005
Plasmid#139754PurposeFor protein expression/crystallography (PDB:6F58)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6ExpressionBacterialMutationContains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only